Fig. 5.

RT-PCR analysis of expression of drought-responsive genes in OsWRKY11-ox and OsWRKY11-kd plants. a Total RNA was isolated from leaves of OsWRKY11-ox plants from lines #73 and #80, and from WT controls. RT-PCR was performed using gene-specific primers against DIP1, DHN1, and RAB21. b OsWRKY11-kd and non-transgenic (WT) control plants were subjected to drought stress. Samples were collected 6 DAT. RT-PCR was performed using gene-specific primers against OsWRKY11, RAB21, DHN1, and DIP1. The transcript level of OsACTIN was used as an internal control. These experiments were repeated more than twice