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. 2018 Jan 12;8:627. doi: 10.1038/s41598-017-18974-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Dissociation equilibrium constants (K_d) for T7 RNA polymerase and the VSR10 dsDNA/RNA heteroduplex. Data shown were obtained from representative assays carried out at pH 7.9 and 6 mM MgCl₂ (panel A) and at pH 6.75 and 1.5 mM MgCl₂ (panel B). The solid line represents the best fit of the data to the quadratic equation relating the template-primer bound to the T7 RNAP and the total concentration of the VSR10 heteroduplex. Reported K_d values represent averages ± standard deviations obtained from at least three independent experiments.