Table 2.
Summary of error rates for WT and mutant RTs, for various classes of mutations in M13mp2 lacZα forward mutation assays.
| Mutation type | HIV-1BH10 RT | AMV RT | MLV RT | HIV-1ESP49 RT | O_K65R RT | O_K65R/V75I RT | ||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|
| No. of errors | Error rate | No. of errors | Error rate | No. of errors | Error rate | No. of errors | Error rate | No. of errors | Error rate | No. of errors | Error rate | |
| All classes | 54 | 1/28524 | 43 | 1/31395 | 46 | 1/35397 | 44 a | 1/36401 | 58 | 1/37829 | 71 | 1/39739 |
| Base substitutions | 26 | 1/36434 | 25 | 1/33210 | 29 | 1/34531 | 19 | 1/51843 | 29 | 1/46530 | 53 | 1/32739 |
| Transitions | 11 (42%) | 10 (40%) | 12 (41%) | 10 (53%) | 19 (66%) | 43 (81%) | ||||||
| Transversions | 15 (58%) | 15 (60%) | 17 (59%) | 9 (47%) | 10 (34%) | 10 (19%) | ||||||
| Frameshifts | 28 | 1/48960 | 18 | 1/66750 | 17 | 1/85245 | 25 | 1/57018 | 29 | 1/67336 | 18 | 1/139505 |
| Insertions | 7 (25%) | 14 (78%) | 9 (53%) | 13 (52%) | 10 (34%) | 9 (50%) | ||||||
| Deletions | 21 (75%) | 4 (22%) | 8 (47%) | 12 (48%) | 19 (66%) | 9 (50%) | ||||||
| At runsb | 10 (36%) | 1/29266 | 10 (56%) | 1/25650 | 7 (41%) | 1/44196 | 12 (48%) | 1/25359 | 13 (45%) | 1/32067 | 12 (67%) | 1/44673 |
| At non-runs | 18 (64%) | 1/59901 | 8 (44%) | 1/118125 | 10 (59%) | 1/113980 | 13 (52%) | 1/86242 | 16 (55%) | 1/95991 | 6 (33%) | 1/329168 |
The RNA used as template in the reverse transcription reaction was synthesized by the T7 RNAP (Promega) in a transcription buffer containing 40 mM Tris-HCl pH 7.9 and 6 mM MgCl2.
aThe total number of errors with this RT was smaller than the number of mutant plaques (Table 1), because five of those mutant plaques were either recombinants or had complex arrays of mutations, and were not included in the analysis of error rates.
bA run is considered when there is a row of three or more identical nucleotides.