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. 2018 Jan 12;8:670. doi: 10.1038/s41598-017-18428-1

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© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Proliferative Response of Breast Cancer Cell Lines to CM from TNF-α/IFN-γ- Induced and Non-Induced Placental-Derived ASCs. (a) Proliferative response of the six breast cancer cell lines to undiluted CM from TNF-α/IFN-γ-induced-ASC in the 3D-spheroid assay. Red bars represent TNBC cell lines. Error bars represent SD (n = 3). (b) POC response curves for MDA-MB-231 in the 3D-spheroid assay upon serial dilution. Error bars represent SD (n = 3). (c) Inhibition of MDA-MB-231 2D growth in real time with CM from TNF-α/IFN-γ-induced and non-induced-ASCs. Error bars represent SEM (n = 4 for MDA-MB-231 with regular growth medium, n = 11 for MDA-MB-231 + induced-ASC CM, n = 8 for MDA-MB-231 + non-induced-ASC CM). P-values are based on ANOVA: ***p < 0.01, **p < 0.01, and *p < 0.05.