Table 2.
Anti-Proliferative Effect of TNF-α/IFN-γ-Induced Placental-Derived ASC CM on Cancer Cell Lines in a 3D-Spheroid Cell Proliferation Assay.
| Cell Line Source | Cell Lines Tested | Strong Positive Response (POC < 60%) | Marginal Positive Response (60% < POC < 80%) | No Effect (80% < POC < 120%) | Negative Response (120% < POC < 150%) |
|---|---|---|---|---|---|
| Bladder | 5 | 0 | 0 | 4 | 1 |
| Brain | 4 | 0 | 1 | 3 | 0 |
| Breast | 6 | 2 | 1 | 0 | 3 |
| Cervix | 1 | 0 | 0 | 0 | 1 |
| Colorectal | 10 | 2 | 1 | 7 | 0 |
| Head/Neck | 1 | 0 | 0 | 1 | 0 |
| Kidney | 4 | 2 | 1 | 1 | 0 |
| Liver | 5 | 2 | 1 | 2 | 0 |
| Lung | 6 | 2 | 2 | 2 | 0 |
| Muscle | 1 | 1 | 0 | 0 | 0 |
| Ovary | 2 | 0 | 1 | 1 | 0 |
| Pancreas | 2 | 0 | 0 | 2 | 0 |
| Pharynx | 1 | 0 | 0 | 1 | 0 |
| Prostate | 4 | 0 | 4 | 0 | 0 |
| Skin | 4 | 1 | 1 | 2 | 0 |
| Stomach | 1 | 0 | 0 | 1 | 0 |
| Thyroid | 1 | 0 | 0 | 1 | 0 |
| Uterus | 1 | 0 | 1 | 0 | 0 |
| TOTAL | 59 | 12 | 14 | 28 | 5 |
| 20% | 24% | 48% | 8% |
Summary of the 3D-spheroid proliferation assay. Results are listed according to the source organ of the cancer cell lines. Within each organ, “percent of cell proliferation relative to control medium” (POC) results are categorized into one of four categories: strong positive response (POC < 60%), marginal positive response (60% < POC < 80%), no effect (80% < POC < 120%) and negative response (120% < POC < 150%).