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. 2018 Jan 12;8:671. doi: 10.1038/s41598-017-18421-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Nuclear low-molecular weight (LMW) β-catenin predominates in colon cancer IEC. Protein isolates from biopsy-derived normal and colorectal cancer (CRC) IEC were probed with antibodies specific for distinct β-catenin epitopes. (A) Normal and CRC IEC fraction lysates (cytosolic (Cyto), membrane (Memb) and nuclear (Nucl) were probed sequentially for β-catenin antibodies directed to different epitopes as shown. Purity controls were achieved by probing for α-tubulin (cytosol), E-cadherin (membrane) and lamin B1 (nucleus). (B) Nuclear lysates from normal and CRC biopsies were probed for phospho-β-catenin^Ser552 (pβ-Cat⁵⁵²). WB for lamin B1 serves as loading control. Arrows indicate positions of LMW β-catenin. Full size membrane scans for WBs can be seen in Suppl. Fig. SS1.