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. 2018 Jan 12;8:671. doi: 10.1038/s41598-017-18421-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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TCF4 binds proteasome-sensitive LMW-β-catenin. (A) IEC nuclear fractions from normal (N) and CRC biopsies were immunoprecipitated by anti-TCF4 and probed for pβ-Cat⁵⁵², core region β-catenin and TCF4. (B) Nuclear fractions from CRC biopsies were used for IP with different β-catenin antibodies. Precipitated proteins were probed with anti-TCF4 antibody. Control WBs with N and C terminal, core region specific and pβ-Cat⁵⁵² antibodies can be seen in Suppl. Fig. S6D. (C) Normal, CRC and CRC treated with MG132 IEC nuclear fraction were immunoprecipitated by anti-TCF4 and probed for pβ-Cat⁵⁵². Input and loading control WBs for these samples can be seen on Suppl. Fig. S6E. (D) Chromatin-bound nuclear fractions of HT29 cell, untreated and treated with bortezomib (input and loading controls in Fig. 2B), were immunoprecipitated by anti-TCF4 and probed for anti-pβ-Cat⁵⁵² and core region β-catenin antibodies. Full size membrane scans for WBs can be seen at Suppl. Fig. SS4.