Table 3.

ZIKV Env peptide relative binding affinity to HLA-DR molecules.

Env peptide	DR1	DR3	DR4	DR7	DR11	DR15	DRB5
p1 [1–20]	638	8	118	86	>2 248	175	474
p14 [131–150]	1	250	0.2	7	0.4	0.2	1
p31 [301–320]	3	>1 129	11	1	50	53	2
p41 [401–420]	900	>1 129	114	>359	4	75	65
p42 [411–430]	319	19	>477	119	5	125	54

Data were expressed as relative affinity: ratio of the half maximal inhibitory concentration of the peptide to the half maximal inhibitory concentration of the reference peptide. The peptide concentration preventing 50% binding of labeled peptide (IC50) was evaluated and data expressed as relative affinity: the ratio of the IC50 of test peptide to the IC50 of reference peptide. Mean ± SEM was calculated from two to three independent experiments. Relative affinities of 10 or less were considered high binders, and relative affinities of 10–100 were moderate binders.