Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 12;8:612. doi: 10.1038/s41598-017-18765-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2017

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

(a) Structure of GFP-tagged Y14 serine-alanine substitution mutants and the RKPL mutant, which mimics dephosphorylated Y14. (b) Expression of transfected GFP-Y14 and serine-alanine substitution mutants was detected by western blotting with an anti-GFP antibody (upper panel). Phosphorylation status of Y14 and mutants was analyzed by separation using phos-tag gels (bottom panel). The samples for SDS-PAGE and phos-tag SDS-PAGE derive from the same experiment. The cropped blots are used in the figure. The membranes were cut prior to exposure so that only the portion of gel containing the desired bands would be visualized. Full-length blots are shown in Supplementary Fig. S10. (c) Localization of GFP-Y14 and its associated mutants was observed by fluorescence microscopy (green). Fibrillarin and nuclear DNA are shown as red and blue, respectively. Bar indicates 20 μm. (d) Expression of transfected GFP-Y14 and RKPL mutant was detected by western blotting with an anti-GFP antibody (left panel). Phosphorylation status of Y14 and mutants was analyzed by separation using phos-tag gels (right panel). (e) Localization of GFP-Y14 and the RKPL mutant was observed by fluorescence microscopy (green). Fibrillarin and nuclear DNA are shown as red and blue, respectively. Bar indicates 20 μm.