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. 2018 Jan 12;8:687. doi: 10.1038/s41598-017-18716-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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c-fos expression in dopaminergic neurons during successful acquisition (SA, N = 8 per group). SPR = single pellet reaching, RC = reward control, CC = cage control. T = trained, U = untrained, L = left, R = right hemisphere. Asterisks indicate significant differences (*p < 0.05, **p < 0.01, ***p ≤ 0.001 in Tukey’s HSD test), values are mean ± s.e.m. (A) Superposition of TH⁺c-fos⁺ (green) and FB⁺ TH⁺c-fos⁺ (pink) neurons in VTA derived from 8 animals (2 sections per position per animal). Numbers indicate position relative to bregma. (B) Number of FB⁺ TH⁺c-fos⁺ neurons in rostral and caudal VTA sections. (C) Number of TH⁺c-fos⁺ neurons in rostral and caudal VTA sections.