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. 2018 Jan 12;8:629. doi: 10.1038/s41598-017-19035-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression levels of IL-1β, IL-6, TNF-α, TLR3 and TLR4 genes in whole embryos and, from the offspring, in blood and brain samples obtained from BTBR and C57BL6/J mice. The cytokines and TLRs expression in whole embryos (panel A–E) at gestational days 10.5, in blood (panel A’–E’) and brain samples (panel A”–E”) at post natal day 1 and 7 from BTBR (red box plots) and C57BL6/J (white box plots) mice, was examined by real time-PCR; depicting mild (black dot) and extreme (asterisk) outliers for each group were showed. In the BTBR embryos and in blood and brain samples, higher levels of cytokines and Toll-like receptors expression than in age-matched C57BL/J controls were detected.