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. 2018 Jan 12;8:618. doi: 10.1038/s41598-017-19128-6

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Most contigs stopped extending due to repeats. (a) Shows the median depth of coverage over the last 60 bp of each contig, the results are separated based on whether the end of that contig continues to extend in iteration 31. The mode depth for extending contigs (dashed line) is used to divide the others into low coverage and repeats. (b) Most contigs stopped extending because of repeats, defined by a high median coverage. (c) The length walked from the nearest mapped exon is shown according to the contig status. (d) The length walked from the outermost exons is shown, irrespective of whether the contig had stopped extending.

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