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. 2018 Jan 12;8:667. doi: 10.1038/s41598-017-18045-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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The crystal structure of muGFP. (A) The crystal structure of muGFP (PDB ID: 5JZL). The two molecules of the asymmetric unit were oriented with the β-barrel axes approximately parallel to one another. The chromophore is shown in stick representation and L69 and Y164 are shown in magenta. (B) L69 of muGFP showing the absence of the water molecule observed near Q69 of sfGFP. (C) Y164 of muGFP. Hydrogen bonds are shown as dashed yellow lines.