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. 2018 Jan 12;8:667. doi: 10.1038/s41598-017-18045-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Confocal imaging and fluorescence intensity quantification of pre- and post-CLARITY cleared sections of mouse cortex expressing EGFP and muGFP. Neurons of the primary somatosensory cortex of four mice were virally transduced with EGFP in the left hemisphere (A and D) and muGFP in the right hemisphere (B and E). Maximum projections from a representative brain of confocal image stacks from a fixed coronal brain section with no clearing, with EGFP transduced neurons (A) exhibiting slightly less fluorescence than muGFP transduced neurons (B). (C) The mean sum intensities of transduced neurons from two brains were calculated, with muGFP exhibiting significantly higher staining intensity. Maximum projections of confocal image stacks from a CLARITY cleared 3 mm section demonstrated that EGFP fluorescence was highly attenuated (D) compared to muGFP (E), which was quantified with sections from two brains (F). Groups were compared using an Exact Mann-Whitney ranksum test for equality of medians and **** indicates the medians were significantly different, p < 0.05.