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. 2018 Jan 12;8:566. doi: 10.1038/s41598-017-18916-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Distribution of the significantly enriched terms/pathways using genes associated to the milk nitrogen fractions. The SNP (P < 0.05) were assigned to genes if they were located within the gene or in a flanking region of 15 kb up- and downstream of the gene using the biomaRt R package. For mapping, the Ensembl Bos taurus UMD3.1 assembly was used as reference. Gene-set enrichment analysis was carried out using the goseq R package. Only the traits showing significantly enriched terms are reported (q < 0.05). (a) GO terms; (b) KEGG-pathways. β-CN: β-casein; CN: Σcaseins (β-CN+ κ-CN+ α_S1-CN+ α_S1phosphorylated-CN+ α_S2-CN+ α_S1 (phosphorylated)/α_S1-CN); Nmin: minor nitrogen compounds; a_S1-CN: α_S1-casein; a_S1P-CN: α_S1(phosphorylated)-casein; κ-CN: κ-casein.