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. 2018 Jan 12;8:688. doi: 10.1038/s41598-017-18874-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fluorescence and light scattering properties of the HSPB1 constructs. (a) Normalized spectra of fluorescence of the WT HSPB1 and its mutants. (b) Completeness of thermal transition, expressed as a fraction of conversion from native to denatured state (α), calculated on the basis of temperature dependence of fluorescence spectra for different HSPB1 species. Data are representative of at least four independent experiments with error bars corresponding to the standard error of the mean. (c) Temperature dependence of light scattering. Data are representative of at least three independent experiments with error bars corresponding to the standard error of the mean. The maximal value of light scattering was taken as 100%. (d) The effect of temperature on the average particle hydrodynamic diameter determined by DLS. Data are representative of at least four independent experiments with error bars corresponding to the standard error of the mean.