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. 2018 Jan 12;8:688. doi: 10.1038/s41598-017-18874-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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SEC-coupled SAXS analysis of WT HSBP1 and the three ACD mutants. For each construct four plots are shown: (а) the averaged scattering curve (circles) and overlayed regularized curve (black line) calculated using data at the elution maxima, (b) the corresponding pair-distribution function, (c) dimensionless Kratky plot of the same data, and (d) the radius of gyration (black crosses, left axis) and normalised forward scattering (circles, right axis) at each elution point. For reference the dimensionless Kratky plot also shows the Guinier approximation of a spherical particle at low angles (black line) plotted using the function f(x) = x² * exp(−x²/3).