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. 2018 Jan 12;8:638. doi: 10.1038/s41598-017-18947-x

Figure 1.

Figure 1

Cellular antisense activity in HeLa pLuc705 cells of PNAs 4305, 4263, 4265, 4306, 2787 and 2534. After transfection of the cells with 3 µM PNA, irradiation of cells was carried out with the excitation wavelength specific for each fluorophore for 1, 5, 10, 20, 25 and 30 min. Cells were incubated further for 24 h after irradiation. All the samples were subjected to the luciferase analysis. Luciferase activities were analysed by Bright-Glo reagent (Promega) and are expressed as relative light units (RLU/well) normalized by the ATP. Each data set represents the mean ± SD of triplicate experiment. CQ: chloroquine. TM-Rhodamine: Tetramethylrhodamine.