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. 2018 Jan 12;8:638. doi: 10.1038/s41598-017-18947-x

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Controlled site specific effect of PCI. Cells were transfected with 3 µM PNA. (a,c) Cell nuclei were stained with Hoechst for 15 min.; (b) Cells transfected with TMR conjugated PNA irradiated at 555 nm; (d) Cells transfected with fluorescein conjugated PNA irradiated at 490 nm. Irradiation was confined to the dotted areas (dotted lines). Fluorescence microscope (Diaplan, leitz) with 20× objective lens was used for irradiation and imaging.