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Fluorescence microscope images of unfixed HeLa pLuc705 cells following treatment with 3 µM TMR (a–c) and AF555 (d–f) conjugated PNAs and 10 µM APF. After 4 h incubation, the cells were washed twice with phosphate buffered saline (PBS), fresh medium was added and the cells were irradiated at 555 nm. Images were taken at 40× magnification using a Diaplan, Leitz fluorescence microscope.
