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. 2018 Jan 12;8:604. doi: 10.1038/s41598-017-19058-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Experimental design and representative evaluation by Toluidine Blue and TEM of the contralateral and reversed-autograft at 12 weeks from surgery. (A) Schematic illustration of the experimental design, consisting in entubulating the proximal and distal stump of the transected sciatic nerve (gap = 5 mm) by a reversed-autograft or a conduit which has to guide the regeneration of nerve fibers. At 12 weeks from surgery, samples were excised and divided into 3 sections (proximal stump, central portion and distal stump) according to transverse planes each of which was analyzed. (B) Representative Toluidine Blue staining image (scale bar = 50 µm; scale bar in upper right insert: 100 µm) and TEM micrograph (scale bar = 2 µm) of the controlateral sciatic nerve of a Sprague-Dawley rat. (C) Toluidine Blue staining (scale bar = 50 µm; scale bar in upper right insert: 100 µm) and TEM micrographs (scale bar = 2 µm) of the proximal, central and distal section of a reversed-autograft at 12 weeks from surgery.