Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 12;8:605. doi: 10.1038/s41598-017-19103-1

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Image processing by HyphaTracker macro for automated data evaluation. Germination of conidia of the CarO-deficient F. fujikuroi strain was recorded with a frame rate of 0.2 min⁻¹ in a 16-bit image time series (HT-teststack1). Three different time points as indicated are given to visualize the processing procedure of the images by HyphaTracker. In the left column 16-bit images are shown after data reduction and drift correction. The hyphae are slightly out of focus to enable dark appearance in the microscope image. In the middle column the result of step 3 (Binary image generation) of HyphaTracker is shown. In step 4 and 5 (right column) the ROIs are identified and filtered for their size and eventually occurring crossing events or contact to the edge. Red arrows highlight such germlings that were automatically removed from the analysis. Black bar represents 100 µm.