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. 2018 Jan 12;8:679. doi: 10.1038/s41598-017-19068-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Induction of citrullinated proteins in mice sera following nickel nanomaterials exposure. (a) Serum samples from nickel nanomaterial-injected mice, vehicle control and untreated mice (N/T) were probed by immunoblotting technique for citrullinated proteins. (b) Densitometric quantitation of citrullinated protein bands indicated a significant increase in protein citrullination due to nickel nanomaterials exposure compared to vehicle and untreated controls. Data are representative of three independent experiments (mean ± S.E.). *p < 0.05 with respect to corresponding controls. Note: western blot gels were adapted to fit within composite image, full gel images are provided in Supplemental Information (Figure S2).