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. 2018 Jan 12;8:679. doi: 10.1038/s41598-017-19068-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Detection of anti-CCP3 antibodies in murine sera. (a) Serum samples from nickel nanomaterial-injected and vehicle-injected mice were examined for anti-CCP3 antibodies by ELISA. (b,c) Reactivity of anti-citrulline antibody was confirmed by indirect immunofluorescence after incubating sera from mice exposed to S-NiNW and vehicle controls on oesophageal tissue sections.