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. 2018 Jan 12;8:679. doi: 10.1038/s41598-017-19068-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical analysis of citrullinated proteins in murine lymphoid organs and kidneys. Mice were injected with PBS (vehicle) or NiNPs, S-NiNWs and L-NiNWs and the organs were analysed after 24 h and 2 weeks. LN and kidney tissue sections were stained with anti-citrulline antibody. Protein citrullination in LN (a) spleen (b) and kidney tissue sections (c) sections were compared to their group controls. p-values of <0.0001 are indicated by ***. Statistical analysis between the different experimental groups is reported in Table S1.