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. 2018 Jan 12;8:679. doi: 10.1038/s41598-017-19068-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical analysis of PAD2 expression in murine lymphoid organs and kidneys. Mice were injected with PBS (vehicle) or NiNPs, S-NiNWs and L-NiNWs for 24 h and 2 weeks. LN and kidney tissue sections were stained with anti-PAD2 antibody. The histograms shown the percentage of positively stained cells for PAD2 expression in LN (a), spleen (b), and kidney tissue sections (c) p-values of <0.0001 are indicated by ***. Statistical analysis between the different experimental groups is reported in Table S2.