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. 2018 Jan 12;8:679. doi: 10.1038/s41598-017-19068-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemistry of anti-citrulline, anti-PAD2 and PAD4 antibodies on murine spleen tissue sections. Immunodetection of citrullinated proteins, PAD2 and PAD4 enzymes in murine spleen tissue sections 2-week post exposure to nickel nanomaterials and vehicle controls. Brown staining demonstrates positive immunohistochemical reaction for citrulline, PAD2 and PAD4. First two panels from left (Citrulline) represent the corresponding microscopic fields at lower (10×) and higher (20×) magnification stained for citrullinated proteins, to illustrate the topography of positive staining within the spleen. Red rectangles indicated by arrows outline the regions of interest magnified in the adjacent panel. Two panels on the right show representative topographically similar, but not identical microscopic fields stained for PAD2 and PAD4 enzymes, respectively. Scale bar (yellow): 100 μm.