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. 2018 Jan 12;8:625. doi: 10.1038/s41598-017-18611-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Differences in the drinking processes among habitats in vertebrates. In tetrapods, systemic angiotensin II evokes thirst to motivate a series of drinking behaviours. Fully aquatic fish can complete drinking without thirst because water available for swallowing is always present in the mouth. Mudskipper fish are amphibious gobies that spend the greater part of their lives out of water and it is intriguing to examine whether thirst followed by a series of drinking behaviours is induced by angiotensin II.