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. 2018 Jan 12;9:168. doi: 10.1038/s41467-017-02585-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — SOCS3 interacts with multiple regions within cavin-1. a An immobilised library of 25-mer peptides sequentially shifted by 5 amino acids along the entire cavin-1 open reading frame was overlaid with either purified SOCS3 or a negative control (Cntrl). Dark spots represent areas of interaction between SOCS3 and peptides within the cavin-1 peptide array. The domain structure of murine cavin-1 is indicated below the overlay. b Schematic representation of the N- and C-terminally truncated myc-tagged cavin-1 mutants used for co-IP experiments. c Protein-equalised soluble cell extracts from HEK293 cells transfected with expression constructs encoding either myc-tagged WT cavin-1 or the indicated truncation mutants and Flag-tagged SOCS3 as indicated were processed by IP with anti-Flag M2-agarose beads prior to SDS-PAGE and immunoblotting with the indicated antibodies. Whole-cell lysates from the same samples used in the IP were also fractionated by SDS-PAGE for immunoblotting in parallel. *Indicates that expression of the C1 cavin-1 mutant was not detectable; Non Spec refers to immunoglobulin-derived non-specific staining