Figure 5.

STAP1 knockdown does not affect common signaling pathways. Expression of 17 proteins was determined using a fluorescent bead-based immunoassay in Nalm6 cells after knockdown of STAP1. Fluorescent intensity values relative to GAPDH are depicted. Expression was measured three and seven days after transduction. Infection and selection for stably transduced cells required three days. Subsequently, proliferation, viability and MTS assays were initiated, which took four days. mRNA and protein expression levels were examined after selection (day 3) and at the end of these functional experiments (day 7). At day 7, not enough material was available of Nalm6 cells, which were transduced with shRNA-2. Values represent mean ± SEM of four independent experiments at three days after transduction and three independent experiments at seven days after transduction.