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. 2018 Jan 12;8:657. doi: 10.1038/s41598-017-19083-2

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Real-time tracking software process. The picture shows the series of computer vision filters for acquiring the target (a), and a sample image of zebrafish tracking in the (b) top (left) and front (right) views. The fish trajectory corresponding to 100 consecutive frames is shown in yellow. Corners of the near and far side of the water tank are marked with green squares and circles, respectively.