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. 2018 Jan 12;8:591. doi: 10.1038/s41598-017-18995-3

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Inkjet-printing based determination of PDE4B inhibition by roflumilast. (A) Represents printing order; (B) fluorescence signal generated on parchment paper as a function of C value; (C) graph represents the average fluorescence intensity of the individual images (4A) as a function of C value; and (D) the X-axis represents the number of mole of roliparm printed per spot, the primary Y-axis represents the FRET signals produced in the presence of cAMP, PDE4B and roflumilast, and the secondary Y-axis represents the ejection volume of roflumilast. Error bars represent the standard deviation of three independent measurements.