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Schematic of oral rotenone administration regimen and measurable outcomes. Male C57BL/6 mice were acclimatized 2 weeks before the experiments. Mice received once daily oral rotenone administration. One group (n = 5) was for in vivo tests (fecal pellet collection, open field test, and pole test) weekly during the period. The other five groups (n = 6–8 per group) were sacrificed at the indicated time point, colon, and brain tissues were collected for biochemical examinations.
