Figure 4.
Minor contribution of defective ribosomal products to the direct MHC class I presentation of GFP-S8L-F12-derived S8L. (A) Timeline of the experiments performed in (B,C). (B,C) BMC-2 cells (B) or MC57G cells (C) were simultaneously treated with 1 µg/mL doxycycline (Dox) and 1 µM degradation tag (dTAG)-7 to induce GFP-S8L-F12 expression and degradation at the same time. Next, GFP-specific fluorescence (MFI-GFP) and MHC class I presentation of GFP-S8L-F12-derived S8L (MFI-H-2Kb/S8L) was detected by flow cytometry after 3 and 6 h, as indicated. MFI, mean fluorescence intensity. (D) Quantification of three independent experiments performed in (B,C) (for calculation details see Materials and Methods). All experiments were performed in triplicates and results are shown as mean ± SEM. (B,C) Representative results from three independent experiments are shown.