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. 2018 Jan 8;11:423. doi: 10.3389/fncel.2017.00423

Figure 5.

Figure 5

Dissociated MGE–derived cells develop into GABAergic interneurons. (A) DIV9 cells stained for axonal marker Tau and dendritic marker MAP2. (B,C) Cells at the indicated DIVs immunostained for different markers of GABAergic interneurons. (D) Left: DIV13 MGE–derived cells cultured on Matrigel were stained for Tau and MAP2. Examples of Tau–positive axons (arrows) and MAP2-positive dendrites (arrowheads) are shown. Right: DIV15 cells on Matrigel immunostained for GABA and DAPI. (E) DIV20 interneurons on Matrigel immunostained for GABAergic presynaptic terminals (VGAT) and inhibitory postsynaptic terminals (gephyrin). Arrows indicate sites of juxtaposition of the two markers. (F,G) DIV9 (F), and DIV10 (G) cells cultured on PLL and LN with or without BDNF. Arrows in (E–G) indicate examples of juxtaposition of VGAT and gephyrin. Bars: 60 μm (A,B); 40 μm (D); 20 μm (C,E,F); 12 μm (G).