Skip to main content
. 2018 Jan 8;11:423. doi: 10.3389/fncel.2017.00423

Figure 6.

Figure 6

BDNF promotes neurite outgrowth from dissociated MGE–derived cells. (A) DIV6 neurons incubated without or with BDNF (50 ng/ml) and immunostained for MAP2 and Tau. Nuclei are revealed by DAPI staining. Each column shows the three stainings of the same field. Bar, 40 μm. (B) Quantification of the total length of neurites per cell. Bars are mean values of the average total length of MAP2-positive and Tau-positive neurites per cell from DIV6 cultures. Bars are mean values ± SEM from 20 fields per experimental condition, with a total of 285 BDNF–treated cells, and 308 untreated cells. **p = 0.00525 (MAP2-positive neurites); ***p = 0.00083 (Tau-positive neurites). (C) Left: examples of inverted images of DIV6 MGE–derived cells cultured with or without BDNF. Bar, 40 μm. Right: Sholl analysis on DIV6 MGE–derived neurons cultured with ±50 ng/ml BDNF; bars are mean values ±SEM from 17 cells per experimental condition. *p < 0.05; **p < 0.01; ***p < 0.001.