Table 1.
Primary antibodies and IgG controls used in this study
| Antibodya | Host | Supplier/Catalog#b | Dilution |
|---|---|---|---|
| GFP | Mouse monoclonal | SCB/sc-32422 | 1:200 (IHC); 1:400 (Wb) |
| GFP | Rabbit polyclonal | CS/2555 | 1:200 (IHC) |
| mCherry | Rabbit polyclonal | LF/PA534974 | 1:200 (IHC); 1:400 (Wb) |
| Hmgcs1 | Mouse monoclonal | SCB/sc-393256 | 1:200 (IHC) |
| Tubb3 | Mouse monoclonal | SCB/sc-80016 | 1:400 (IHC) |
| Vimentin | Goat polyclonal | SCB/sc-12886R | 1:600 (IHC) |
| GFAP | Rabbit polyclonal | Dako/Z0334 | 1:1000 (IHC); 1:2000 (Wb) |
| GS | Rabbit polyclonal | SCB/sc-6640R | 1:600 (IHC) |
| Iba1 | Rabbit polyclonal | Wako/019-19741 | 1:1000 (IHC) |
| Kir4.1 | Guinea pig polyclonal | AlomoneLabs/AGP-012 | 1:100 (IHC) |
| EAAT1 | Rabbit polyclonal | AlomoneLabs/AGC-021 | 1:100 (IHC) |
| S100 | Mouse monoclonal | NeoMarkers/MS296Pl | 1:200 (IHC) |
| MBP | Goat polyclonal | SCB/sc-13912 | 1:1000 (IHC) |
| GAPDH | Mouse monoclonal | PT/2555 | 1:2000 (Wb) |
| CD6 | Mouse monoclonal | Millipore/CBL554 | 1:100 (IHC) |
| CD8 | Mouse monoclonal | Millrpore/217580 | 1:100 (IHC) |
| IgG control | Mouse | LF/31903 | 1:100~400 |
| IgG control | Goat | LF/31245 | 1:500~1000 |
| IgG control | Rabbit | LF/MA5-16384 | 1:200~1000 |
Antibody abbreviations: Hmgcs1, HMG CoA synthase 1; Tubb3, β3-Tubulin; GFAP, Glial fibrillary acidic protein; GS, Glutamine synthetase; Iba1, Ionized calcium binding adaptor molecule 1; Kir4.1, ATP-sensitive inward rectifier potassium channel 10; EEAT1, Excitatory amino acid transporter 1; S100, S-100 calcium-binding protein; MBP, Myelin basic protein; GAPDH, Glyceraldehyde 3-phosphate dehydrogenase; CD6, Cluster of differentiation 6; and CD8, Cluster of differentiation 8.
SCB, Santa Cruz Biotechnology, Santa Cruz, CA; CS, Cell signaling, Danvers, MA; LF, life Technologies, Carlsbad, CA; Dako, Carpintena, CA; Wako, Richmond, VA; Alomone Labs, Jerusalem, Israel; NeoMarkers, Fremont, CA; PT, Proteintech, Rosemont, IL; Millipore, Billenca, MA.