Fig. 1.

BG promotes myogenesis in C2C12 myoblasts. (A) BG-treated C2C12 cells were differentiated in differentiation medium for 2 d and analyzed by MHC immunostaining. DAPI was used to visualize nuclei. (B) The MHC-positive myocytes shown in panel A were quantified as a number of nuclei per myotube. Values are represented as means ± SD of three independent experiments. **p < 0.01. (C) MHC, MyoD, and myogenin were analyzed by immunoblotting using prepared cell lysates from panel A. (D) The signal intensity of indicated muscle-specific proteins was quantified in three independent experiments and normalized to pan-Cadherin. Values are represented as means ± SD. **p < 0.01. BG, black ginseng; DAPI, 4′,6-diamidino-2-phenylindole stain; MHC, major histocompatibility complex; SD, standard deviation.