Figure 3.

Single turnover deacetylation of singly-acetylated H3 octamers. A, dependence of the apparent deacetylation rate constant of H3K9ac octamer on the concentration of HDAC8. Data points are from multiple measurements in a single reaction at each HDAC8 concentration, and error bars on k_obs values represent errors calculated from the exponential fits. A linear fit of the data indicates that the k_max/K_½ is 3,700 ± 100 m⁻¹ s⁻¹. B, dependence of the apparent deacetylation rate constant of H3K14ac octamer on the concentration of HDAC8. The data points are from multiple measurements in a single reaction at each HDAC8 concentration. A hyperbolic fit indicates that the k_max/K_½ is 1,000 ± 200 m⁻¹ s⁻¹ with a k_max value of 0.03 ± 0.02 s⁻¹.