Figure 4.
Single turnover deacetylation of singly-acetylated H3 nucleosome. The initial rate of progress curves for deacetylation of H3K9ac nucleosome catalyzed by 0–7.5 μm HDAC8 was fit linearly, and the rate constant was calculated assuming 100% deacetylated product. The data points are from multiple measurements in a single reaction at each HDAC8 concentration, and error bars represent errors calculated from the initial rate fits. A linear fit of the data indicates that kmax/K½ is 28 ± 3 m−1 s−1.