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Catalytic efficiencies for deacetylation of histone substrates by HDAC8
HDAC8 activity was measured, and catalytic efficiencies were determined as described under “Experimental procedures” and the legends of Figs. 2–4.
| Substrate | 7-mer peptide | 13-mer peptide | 17-mer peptide | Tetramer | Octamer | Nucleosome |
|---|---|---|---|---|---|---|
| kcat/Km (m−1 s−1) | kmax/K½ (m−1 s−1) | |||||
| H3K9ac | 56 ± 6 | 51 ± 3 | 120 ± 11 | >17,000 | 3,700 ± 100 | 28 ± 3 |
| H3K14ac | 8.0 ± 0.7 | 21 ± 4 | 2,500 ± 70 | 1,000 ± 200 | ||
| H3K56ac | 78 ± 8 | 100 ± 10 | 4,000 ± 600 | |||
