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. 2018 Jan 11;172(1-2):147–161.e12. doi: 10.1016/j.cell.2017.11.034

Figure 3.

Figure 3

Single-Cell Transcriptional Analysis in LT-HSCs upon β-Glucan Administration

(A–C) Single-cell qPCR in LT-HSCs isolated from mice at 24 hr after administration of PBS or β-glucan (n = 42 cells per condition).

(A and B) Hierarchical clustering analysis (A) and distribution of LT-HSCs in the three identified clusters (B) at 24 hr after the administration of PBS or β-glucan.

(C) Violin plots indicating genes with significantly altered expression between clusters 1 and 2. The y axis represents gene expression. The horizontal width of the plot shows the density of the data along the y axis. Color key represents the percentage of cells that express the specific gene.

(D and E) Single-cell qPCR was performed in CD41− and CD41+ LT-HSCs isolated from mice at 24 hr after the administration of PBS or β-glucan. Hierarchical clustering analysis (D) and violin plots indicating genes with significantly altered expression between CD41+ LT-HSCs from PBS and β-glucan-treated mice (E).