Figure S4.

Transcriptional Alterations in Hematopoietic Progenitors 28 Days after β-Glucan Administration, Related to Figure 5

LT-HSCs and MPPs were sorted from mice on day 28 after β-glucan or PBS administration and RNA sequencing was performed (n = 4 mice per group).

(A and C) Differential gene expression in (A) LT-HSCs and (C) MPPs; volcano plots depicting the distribution of the adjusted p values (-log(P-adj.)) and the fold changes (log2 Fold Change). Significant changes are colored red (FDR = 0.05).

(B and D) Overrepresented canonical pathways showing genes that were upregulated (red) or downregulated (blue) in (B) LT-HSCs and (D) MPPs from β-glucan–injected mice, as compared to PBS-treated mice.

(E) Heatmap depicting the expression of lymphoid lineage-related genes in LT-HSCs and MPPs from β-glucan injected mice, as compared to PBS treated mice. Transcripts that are not significantly altered are shown in gray.

(F) Differentially expressed genes in both LT-HSCs and MPPs. The number of upregulated (red) and downregulated (blue) genes is shown.