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Engineered of loop and other regions in CH2 presented by PyMOL. (A) Introduction of an enhanced aromatic sequon (EAS) (Q295F/Y296A) in CH2 loop DE [PDB 3AVE (47)]. The mutated residues are colored by red, whereas the oligosaccharides are colored by lemon color. (B) Truncation of N-terminus of CH2. Seven unstructured residues from A244 to G250 are colored by red, which could be removed [PDB 1HZH (51)].
