Figure 4.
Dose-dependent effect of eel Kiss peptides on pituitary lhβ (A), fshβ (B), and gnrh-r2 (C) expression by eel pituitary cells in primary culture. Pituitary cells were treated with various concentrations (10−11, 10−9, and 10−7 M) of eel Kp1(10), Kp2(10), Kp1(15), and Kp2(12) for 10 days. Pituitary mRNA levels were quantified by quantitative real-time PCR. Data were normalized against β-actin. This figure displays the results of a representative experiment. Each point represents mean ± SEM from five-well replicates. *P < 0.05, **P < 0.01, and ***P < 0.001 versus controls, ANOVA.