Table 1.

Primers used in the 3′- and 5′-RACE PCR and quantitative real-time PCR (qPCR) amplifications.

Primers	5′–3′ sequence (bp)
Primers for 3′-RACE PCR
Kiss1-F	CGCCACAAGCGGCCAAGAAG
Kiss2-F	AGGGCCACATTTCCTGCCGACT
Primers for 5′-RACE PCR
Kiss1-R	CCCGCTTCTTGGCCGCTTGT
Kiss2-R	CCGAACGGATTGCGGTTGAATTTG
Primers for qPCR
Kiss1-F	GGTCTCTTAGGTACACCCCGT	This study
Kiss1-R	ACAGCTCCTCGCTCATTTG
Kiss2-F	ACGGACGACTCAGGTTCTCT	This study
Kiss2-R	GCCCTCGATTTCACTGTCTT
actin-F	AGTATTTGCGCTCGGGTG	Aroua et al. (27)
actin-R	CAGCCTTCCTTCCTGGGT
lhβ-F	TCACCTCCTTGTTTCTGCTG	Aroua et al. (27)
lhβ-R	TAGCTTGGGTCCTTGGTGATG
fshβ-F	TCTCGCCAACATCTCCATC	Aroua et al. (27)
fshβ-R	AGAATCCTGGGTGAAGCACA
gnrh-r1a-F	TGGTCATGAGTTGCTGCTACA	Penaranda et al. (25)
gnrh-r1a-R	AGACACCCCTCTCCGTCTTT
gnrh-r1b-F	TCGTCACGCTCTACGTTGTC	Penaranda et al. (25)
gnrh-r1b-R	AGGCAGGACTCTCCACCTTT
gnrh-r2-F	TCACCTTCTCCTGCCTCTTC	Penaranda et al. (25)
gnrh-r2-R	TTGGAAGATGCCTTCCCTTT