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Phosphomimicking mutants of KIF21A impair their bindings to KANK1 ANK. A, consensus phosphorylation sites (PKA and Akt) located in the KBD fragment of KIF21A. B–D, ITC-based measurements of binding affinities between the ANK domain of KANK1 and different phosphomimicking mutants of KIF21A KBD.
