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. 2017 Nov 6;293(1):89–99. doi: 10.1074/jbc.M117.801019

Figure 3.

Figure 3.

Sedimentation velocity c(s) profiles of wild-type and mutants of 14-3-3ζ at 0.27 mg/ml (A) and 0.09 mg/ml (B): 14-3-3ζ wild type (solid line), S58D (dotted line), D21N (dashed line), and E89Q (gray line). The position of the 3.75 S dimeric species is indicated by the open arrow, and that of the 6.20 S tetrameric species is shown by the solid black arrow (in B only). The insets show expansions of the profiles for the indicated regions. In A, root mean square deviation for fit of wild-type 14-3-3ζ is 0.00627, and the values for S58D, D21N, and E89Q are 0.00507, 0.00577, and 0.00544 with run test z values of 5.42, 7.15, 14.6, and 11.82 respectively. B, root mean square deviation for fit of wild-type 14-3-3ζ is 0.00471, and the values for S58D, D21N, and E89Q are 0.00472, 0.00457, and 0.00485 with run test z values of 3.71, 1.12, 0.08, and 1.97, respectively.