Table 2.
Secondary structure of recombinant wild-type and mutant 14-3-3ζ proteins
CD data were analyzed using CDPro (sites.bmb.colostate.edu/sreeram/CDPro) with the CONTINLL algorithm (21) and compared with data in the SP43 reference database. (Please note that the JBC is not responsible for the long-term archiving and maintenance of this site or any other third party hosted site.)
| 14-3-3ζ protein | Composition |
||||
|---|---|---|---|---|---|
| α-Helix | β-Sheet | Turn | Unordered | Root mean square deviation | |
| % | % | % | % | % | |
| WT | 79.3 | 0.7 | 6.1 | 13.9 | 0.12 |
| D21N | 63.2 | 5.5 | 10.6 | 20.6 | 0.09 |
| E89Q | 74.8 | 1.7 | 7.7 | 15.8 | 0.11 |
| S58D | 67.2 | 4.1 | 10.3 | 18.5 | 0.08 |