JNK2, but not JNK1, positively regulates HIF-1α in vitro.
A, JNK1 and JNK2 immunoblots from WT, Jnk2−/−, or Jnk1−/− MEFs. B, HIF-1α immunoblots from WT, Jnk1−/−, or Jnk2−/− MEFs treated with hypoxia for 0 min, 15 min, 30 min, 60 min, 2 h, 3 h, 4 h, or 6 h. C, HIF-1α and JNK2 immunoblots from WT MEFs transfected with control siRNA or JNK2-specific siRNA and then treated with hypoxia for 0 min or 2 h. D, HIF-1α and JNK1 immunoblots from WT MEFs transfected with control siRNA or JNK1-specific siRNA and then treated with hypoxia for 0 min, 30 min, 60 min, or 2 h. All immunoblots are representative of at least three independent experiments that yielded similar results.